RESEARCH INTERESTS

• Drug delivery
• Gene therapy
• Liposomes
• Membrane fusion
• Nucleic acid stability
• Transgene expression in the brain
• Targeted drug delivery
• Macromolecule formulation
• Macrophage biology
• Bioterrorism
• Topical delivery

• Liposome formulation
• Delivery of nucleic acids
• Tissue culture
• Disposition of macromolecules
• Membrane fusion via surfactants
• Mycobacteria biology

• Cygene

1. Houk B.D., Martin R., Hochhaus G., Hughes J.A.,  "Pharmacokinetics or Plasmid DNA in the Rat." Pharm. Res., 18:1-10 (2001).
2. Chacham P., Hughes J.A.  “Relationship Between Plasmid and Topological Forms and In Vitro Transfection.”  J. Liposome Res., 11:139-152 (2001).
3. Wu P., Xiao W., Conlon T., Hughes J.A., Agbandje-McKenna M., Ferkol T., Flotte T., Muzyczka N.  “Mutational Analysis of the Adeno-Associated Virus Type 2 (AAV2) Capsid Gene and Construction of AAV2 Vectors with Altered Tropism.”  Journal of Virology, 74:8635-8647 (2000).
4. Liang E., Rosenblatt M.N., Ajmani P.S., Hughes J.A., "Biodegradable pH-sensitive Surfactants (BPS) in Liposome-mediated Nucleic Acid Cellular Uptake and Distribution."  Eur. J. Pharm. Sci., 11:199-205 (2000).
5. Wang S., Bui V., Hughes J.A., King M.A., Meyer E.M.  "Adenoassociated Virus Mediated Gene Transfer into Primary Rat Brain Neuronal and Glial Cultures: Enhancement with the pH-sensitive Surfactant Dodecyl 2-(1'-imidazolyl)propionate.”  Neurochemistry International, 4:1-6 (2000).