Margaret O. James Ph.D.
Box 100485, JHMHC
College of Pharmacy
1600 S.W. Archer Road, Room P6-20
University of Florida
Gainesville, FL 32610-0485
Email: james@cop.ufl.edu
Phone: 352 273 7707
Fax: 352 846 1972
Department Chairman, Jack C. Massey Professor of Pharmacy
B.Sc., Ph.D., D.Sc., University of London, UK, 1969, 1972, 1993
BROAD RESEARCH INTERESTS are factors affecting the metabolism and toxicity of drugs and other xenobiotics in humans and animal species, including aquatic species. This laboratory studies mechanisms of uptake, biotransformation, excretion and toxicity of xenobiotics, especially environmental chemicals. Another interest is the influence of xenobiotics on the metabolism of hormones. Techniques used include enzyme assays, analytical chemistry, enzyme purification, molecular biology, radiochemistry. Specific ongoing research projects are as follows:
I. Biotransformation and bioavailability of ingested xenobiotics. The extent of uptake of ingested xenobiotics into the animal body is influenced by biotransformation in the intestine and liver, as well as transport across the intestinal cells and from liver to bile or blood. Intestinal and hepatic biotransformation may result in either the activation or conversely the detoxication of xenobiotics. Xenobiotics of interest include chemicals such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, organochlorine pesticides and their metabolites and other environmental chemicals. See publications 102-107, 118, 124-126, 132-133.
II. The metabolism of dichloroacetic acid in vivo and in vitro in humans and animal models. Dichloroacetic acid is both an orphan drug, used to lower lactic acid, and an environmental pollutant found in chlorinated drinking water. The first step in metabolism of dichloroacetic acid is glutathione-dependent dehalogenation to glyoxylate, catalyzed by an enzyme whose normal function is in the tyrosine catabolic pathway. As well as the studying the biotransformation of dichloroacetate, we are interested in understanding the toxicological effects of exposure to this agent. See publications 93-97, 111, 114, 123 and 135
III. Effects of xenobiotics on estrogen biotransformation and transport. Recent research has shown that several xenobiotics can affect the biotransformation and transport of estradiol, estrone and other hormones. This may result in beneficial or adverse effects on the organism, depending on the pathway affected. See publications 120, 122, 127
BIBLIOGRAPHY
Refereed publications.
1. Reidenburg, M.M., James, M.O., and Dring, L.G.: The rate of procaine hydrolysis in serum of normal subjects and diseased patients. Clin. Pharmacol. Ther. 13: 279 - 284, 1972.
2. James, M.O., Smith, R.L., Williams, R.T., and Reidenburg, M.: The conjugation of phenylacetic acid in man, subhuman primates and some nonprimate species. Proc. R. Soc. Lond. B. 182: 25 - 35, 1972.
3. James, M.O., Smith, R.L., and Williams, R.T.: The conjugation of 4-chloro- and 4-nitro-phenylacetic acid in man, monkey, and rat. Xenobiotica 2: 499 - 506, 1972.
4. James, M.O. and Smith, R.L.: The conjugation of phenylacetic acid in phenylketonurics. Europ. J. Clin. Pharmacol. 5: 243 - 246, 1973.
5. James, M.O., Bend, J.R., and Fouts, J.R.: Studies on the fate of phenylacetic acid in some fish species. Bull. Mt. Desert Island Biol. Lab. 13: 59 - 62, 1973.
6. James, M. O., Fouts, J.R. and Bend, J.R.: In vitro epoxide metabolism in some marine species. Bull. Mt. Desert Island Biol. Lab. 14: 41 - 46, 1974.
7. James, M.O., Fouts, J.R., and Bend, J.R.: Hepatic and extrahepatic in vitro metabolism of an epoxide (8-14C-styrene oxide) in the rabbit. Biochem. Pharmacol. 25: 187 - 193, 1976.
8. James, M. O., Pohl, R.J., Peret, D.G., Fouts, J.R., and Bend, J.R.: Further studies on epoxide metabolism in vitro by marine species. Bull. Mt. Desert Island Biol. Lab. 15: 46 - 48, 1975.
9. Bend, J.R., James, M.O., Devereux, T.R., and Fouts, J.R.: Toxication-detoxication systems in hepatic and extrahepatic tissues in the perinatal period. In Morselli, P.L., Garattini, S. and Sereni, F. (eds). Basic and Therapeutic Aspects of Perinatal Pharmacology. New York, Raven Press pp 229 - 243, 1975
10. James, M.O. and Bend, J.R.: Taurine conjugation of 2,4-dichlorophenoxyacetic acid and phenylacetic acid as a major metabolic pathway in two marine species. Xenobiotica 6: 393 - 398, 1976.
11. Ryan, A.J., James M.O., Ben-Zvi, A., Law, F.C.P., and Bend, J.R.: Hepatic and extrahepatic metabolism of 14C-styrene oxide. Environ. Hlth. Persp. 17: 136 - 144, 1976.
12. Harper, C., James, M.O., Devereux, T.R., Patel, J.M., Bend, J.R. and Fouts, J.R.: Characteristics and development of drug metabolism by pulmonary microsomes. Agents and Actions 6: 527 - 530, 1976.
13. James, M.O., Fouts, J.R., and Bend, J.R.: Hepatic and extrahepatic in vitro metabolism of an epoxide (8-14C-styrene oxide) in the rabbit. Biochem. Pharmacol. 25: 187 - 193, 1976.
14. Philpot, R.M.,James, M.O. and Bend, J.R.: Metabolism of benzo(a)pyrene and other xenobiotics by microsomal mixed-function oxidases in marine species. Institute of Biological Sciences Symposium. In Sources, Effects and Sinks of Petroleum in the Aquatic Environment. Washington D.C. pp 184 - 199, 1976.
15. James, M.O., Foureman, G.L., Law, F.C.P., and Bend, J.R.: Perinatal development of epoxide hydrase and glutathione S-epoxide transferase in hepatic and extrahepatic tissues of the rabbit and guinea pig. Drug Metab. Dispos. 5: 19 - 28, 1977.
16. Bend, J.R., James, M.O. and Dansette, P.M.: In vitro metabolism of xenobiotics in some marine animals. Ann. N. Y. Acad. Sc. 298: 505 - 521, 1977
17. Guarino, A.M., James, M.O. and Bend, J.R.: Fate and distribution of the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) in the dogfish shark. Xenobiotica 7: 623 - 631, 1977.
18. James, M.O. and Bend, J.R.: Xenobiotic metabolism in marine species exposed to hydrocarbons. EPA Decision Series: Energy/Environment II Environmental Protection Agency US Gov't Press 600/9/77-01 1977, pp 495 - 501
19. James, M.O., Fouts, J.R. and Bend, J.R.: Xenobiotic metabolizing enzymes in marine fish. In Khan, M.A.Q. (ed). Pesticides in the Aquatic Environment. Plenum Press, New York, pp 171 - 189, 1977
20. James, M.O. and Bend, J.R.: A radiochemical assay for glycine N-acyltransferase activity: some properties of the enzyme in rat and rabbit. Biochem. J. 172: 285 - 291, 1978.
21. James, M.O., and Bend, J.R.: Perinatal development of, and effect of chemical pretreatment on, glycine N-acyltransferase activities in liver and kidney of rabbit and rat. Biochem. J. 172: 292 - 299, 1978.
22. Bend, J.R., Foureman, G.L. and James, M.O.: Partially induced hepatic mixed-function oxidase systems in individual members of certain marine species from coastal Maine and Florida. In Hutzinger, O., Van Lelyveld, I.H. and Zoetman, B.L.J. (eds): Aquatic Pollutants. Oxford, Pergamon Press pp 483 - 486, 1978
23. Pritchard, J.B. and James, M.O.: Determinants of the renal handling of 2,4-dichloro-phenoxyacetic acid (2,4-D) by winter flounder. J. Pharmacol. Exp. Ther. 208: 280 - 286, 1979.
24. Pritchard, J.B., Cotton, C.U., James, M.O., Giguere, D. and Koschier, F.J.: Role of metabolism and transport in the excretion of phenylacetic acid and 2,4-dichlorophenoxyacetic acid by marine fish. Bull. Mt. Desert Island Biol. Lab. 18: 58 - 60, 1978.
25. James, M.O., Bowen, E.R., Dansette, P.M. and Bend, J.R.: Epoxide hydrase and glutathione S-transferase activities with selected alkene and arene oxides in several marine species. Chem. Biol. Interact 25: 321 - 344, 1979.
26. James, M.O.: Taurine conjugation of carboxylic acids in some marine species. In Aitio, A. (ed). Conjugation reactions in drug biotransformations. Elsevier/North Holland, Amsterdam. pp 121 - 318, 1979
27. James, M.O., Khan, M.A.Q. and Bend, J.R.: Hepatic microsomal mixed-function oxidase activities in several marine species common to coastal Florida. Comp. Biochem. Pharmacol. 62C: 155 - 164, 1979.
28. Bend, J.R., Ball, L.M., Elmamlouk, T.H., James, M.O. and Philpot, R.M.: Microsomal mixed-function oxidation in untreated and polycyclic aromatic hydrocarbon treated marine fish. In Khan, M.A.Q., Lech, J.J. and Menn, J.J. (eds) American Chemical Society Symposium Series 99. Pesticide and Xenobiotic Metabolism in Aquatic Organisms. pp 297 - 318, l979.
29. James, M.O., and Bend, J.R.: Polycyclic aromatic hydrocarbon induction of cytochrome P-450 dependent mixed-function oxidases in marine fish. Toxicol. Appl. Pharmacol. 54: 117 - 133, 1980.
30. James, M.O. and Little, P.J.: Characterization of cytochrome P-450 dependent mixed-function oxidation in the spiny lobster. Panulirus argus. In J-Å. Gustafsson et al. (eds). Biochemistry, Biophysics and Regulation of Cytochrome P-450. Elsevier/North Holland, pp 113 - 120, 1980.
31. Bend, J.R., James, M.O., Little, P.J. and Foureman, G.L.: In vitro and in vivo metabolism of benzo(a)pyrene by selected marine crustacean species. In Dawe, C.J. et al. (eds). Phyletic Approaches to Cancer. Japan Sci. Soc. Press Tokyo. pp 179 - 194, 1981.
32. James, M.O. and Little, P.J.: Polyhalogenated biphenyls and phenobarbital: Evaluation as inducers of drug-metabolizing activities in the sheepshead, Archosargus probatocephalus. Chem. Biol. Interact. 36: 229 - 248, 198l.
33. Little, P.J., James, M.O., Bend, J.R. and Ryan, A.J.: Imidazole derivatives as inhibitors of cytochrome P-450 dependent oxidation and activators of epoxide hydrolase in hepatic microsomes from a marine fish. Biochem. Pharmacol. 30: 2876 - 2880, 1981.
34. James, M.O.: Disposition and taurine conjugation of 2,4-D, 2,4,5-T, DDA and phenylacetic acid in the spiny lobster, Panulirus argus. Drug Metab.Disp. 10: 516 - 552, 1982.
35. James, M.O. and Bend, J.R.: Effect of polynuclear aromatic hydrocarbons and polyhalogenated biphenyls on hepatic mixed-function oxidase activity in marine fish. Environmental Protection Agency, US Gov't Press 600/9-82-013, 1982, pp 172 - 190
36. James, M.O., Sherman, B., Fisher, S.A. and Bend, J.R.: Benzo(a)pyrene metabolism in reconstituted monooxygenase systems containing cytochrome P-450 from lobster (Homarus americanus) hepatopancreas fractions and NADPH cytochrome P-450 reductase from pig liver. Bull. Mt. Desert Isl. Biol. Lab 22: 37 - 39, 1982.
37. James, M.O. and Little, P.J.: Modification of benzo(a)pyrene metabolism in hepatic microsomes from untreated and induced rats by imidazole derivatives which inhibit monooxygenase activity and enhance epoxide hydrolase activity. Drug Metab. Disp. 11: 350 - 354, 1983.
38. James, M.O. and Little, P.J.: 3-Methylcholanthrene does not induce in vitro xenobiotic metabolism in spiny lobster hepatopancreas, or affect in vivo disposition of benzo(a)pyrene. Comp. Biochem. Physiol. 78C: 241 - 245, l984.
39. Little, P.J., James, M.O., Pritchard, J.B. and Bend, J.R.: Benzo(a)pyrene metabolism in hepatic microsomes from untreated and methylcholanthrene treated southern flounder, Paralichthyes lethostigma. J. Environ. Pathol. Toxicol. Oncol. 5: 309 - 320, 1984.
40. James, M.O. and Shiverick, K.T: Cytochrome P-450 dependent oxidation of progesterone, testosterone and ecdysone in the spiny lobster, Panulirus argus. Arch Biochem. Biophys. 233: 1 - 9, 1984.
41. James, M.O.: Catalytic properties of cytochrome P450 in hepatopancreas of the spiny lobster, Panulirus argus. Marine Environmental Res. 14: 1 - 13, 1984.
42. Little, P.J., James, M.O., Pritchard, J.B. and Bend, J.R.: Temperature dependent disposition of 14C-benzo(a)pyrene in the spiny lobster, Panulirus argus. Toxicol. Appl. Pharmacol. 77: 325 - 333, 1985.
43. James, M.O. and Sloan, K.B.: Structural features of imidazole derivatives which enhance styrene oxide hydrolase activity in rat hepatic microsomes. J. Medicinal Chem. 28: 1120 - 1124, 1985.
44. Stegeman, J.J. and James, M.O. Individual variation in patterns of benzo(a)pyrene metabolism in the marine fish scup (Stenotomus chrysops). Mar. Env. Res. 17: 122 - 124, 1985.
45. Little, P.J., James, M.O., Foureman, G.L., Weatherby, R.P. and Bend, J.R.: 1-14C-n- hexadecane disposition in the spiny lobster, Panulirus argus and the American lobster, Homarus americanus. J. Environ. Pathol. Toxicol. Oncol. 6: 13 - 29, 1986.
46. James, M.O.: Overview of in vitro metabolism of drugs by aquatic species. Human and Vet. Toxicol. 28: 2 - 7, 1986.
47. James, M.O.: Xenobiotic conjugation in fish and other aquatic species. In J.J. Menn, J. Caldwell, D. Hutson and G. Paulsen, eds. Xenobiotic Conjugation Chemistry. American Chemical Society Symposium Series. No. 299, 1986. pp 29 - 47.
48. Riley, C.M. and James, M.O. The analysis of ketoconazole in the plasma, liver, lungs and adrenals of the rat by high performance liquid chromatography. J. Chromatography 377: 287 - 294, 1986.
49. Shiverick, K.T., Swanson, C., Salhab, A.S. and James, M.O. Differential induction of ethoxyresorufin O-deethylase in tissues of the rat placenta. J. Pharmacol. Exp. Therap. 238: 1108 - 1113, 1986.
50. Salhab, A.S., James, M.O., Wang, S-L., and Shiverick, K.T. Positional metabolism of benzo(a)pyrene in rat placenta and maternal liver: Comparison of induction effects. Drug Metab. Disp. 14: 471 - 476, 1986.
51. Shiverick, K.T., Kvello Stenstrom, A.G., Donnelly, W.H., Salhab, A.S., Goldstein, J.A., and James, M.O. Induction of cytochrome P-450 C in hematopoietic cells of fetal liver. Biochem. Biophys. Res. Commun. 141: 299 - 305, 1986.
52. Salhab, A.S., James, M.O., Wang, S-L and Shiverick, K.T. Formation of benzo(a)pyrene DNA adducts by microsomal enzymes: comparison of maternal and fetal liver, fetal hematopoietic cells and placenta. Chem.-Biol. Interact. 61: 203 - 214, 1987.
53. James, M.O.: Conjugation of organic xenobiotics in aquatic animals. Env. Hlth. Persp. 71: 97 - 103, 1987.
54. James, M.O. and Pritchard, J.B.: In vivo and in vitro renal metabolism and excretion of benzoic acid by a marine teleost, the southern flounder. Drug Metab. Disp. 15: 665 - 670, 1987.
55. James, M.O., Heard, C.S. and Hawkins, W.E. Effect of 3-methylcholanthrene on mono-oxygenase activities, epoxide hydrolase and glutathione S-transferase activities in small estuarine and freshwater fish. Aquatic Toxicol. 12: 1 - 15, 1988.
56. Barron, M.G., Gedutis, C. and James, M.O. Pharmacokinetics of sulfadimethoxine in the lobster, Homarus americanus following intrapericardial administration. Xenobiotica, 18: 269 - 276, 1988.
57. James, M.O. Acute and chronic effects of miconazole nitrate on hepatic styrene oxide hydrolase and cytochrome P-450 dependent monooxygenase activities in male and female AKR/J mice. Toxicology, 50: 269 - 281, 1988.
58. Barron, M.G. and James, M.O. Fate of sulfadimethoxine in the lobster, Homarus americanus, Mar. Env. Res., 24: 85 - 88, 1988.
59. James, M.O., Barron, M. G. and Schell, J.D. Conjugation and excretion of phenolic compounds by the lobster, Homarus americanus, Bull. MDIBL. 27: 9 - 11, 1987 - 1988.
60. James, M.O. and Barron, M. G. Disposition of sulfadimethoxine in the lobster. Vet. Human Toxicology, 30: Supplement 1, 36 - 40, 1988.
61. James, M.O.: Conjugation and excretion of xenobiotics by fish and aquatic invertebrates. In, R. Kato, R.W. Estabrook and M.N. Cayen eds. Xenobiotic metabolism and disposition. Taylor and Francis, U.K., USA, 1989. pp 283 - 290.
62. Schell, J.D. and James, M.O. Glucose and Sulfate Conjugation of Phenolic Compounds by the Spiny Lobster, Panulirus argus. J. Biochem. Toxicol. 4: 133 -138, 1989.
63. James, M.O., Schell, J.D. and Magee, V. Bioavailability, biotransformation and elimination of benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol in the lobster, Homarus americanus. Bull. MDIBL, 28: 119 - 121, 1989.
64. James, M.O., Cytochrome P450 monooxygenases in crustacea. Xenobiotica, 19: 1063 - 1076, 1989.
65. Plakas, S.M. and James, M.O. Bioavailability, tissue disposition and renal excretion of benzoic acid in the channel catfish (Ictalurus Punctatus). Drug Metabolism Disp.18: 552-556, 1990
66. James, M.O. Isolation of cytochrome P450 from hepatopancreas microsomes of the spiny lobster, Panulirus argus, and determination of catalytic activity with NADPH cytochrome P450 reductase from vertebrate liver. Arch. Biochem. Biophys. 282: 8 - 17, 1990.
67. James, M.O., Schell, J.D., Barron, M.G. and Li, C-L. J. Rapid, dose-dependent elimination of phenol across the gills, and slow elimination of phenyl sulfate in urine of the lobster, Homarus americanus. Drug Metab. Disp. 19: 536-542, 1991
68. James, M.O. and Pritchard, J.B.: Pesticide metabolism in aquatic organisms. In H. Frehse, ed. Pesticide Chemistry, Advances in International Research, Development and Legislation. VCH Weinheim, Germany, New York, USA, 1991. pp 277-286.
69. James, M.O., Schell, J.D., Boyle, S.M., Altman, A. and Cromer, E. A. Southern flounder hepatic and intestinal metabolism and DNA binding of benzo(a)pyrene (BaP) metabolites following dietary administration of low doses of BaP, BaP-7,8-dihydrodiol or a BaP metabolite mixture. Chemico-Biol. Interact.79: 305-321, 1991.
70. James, M.O., Altman, A.H., Chung-Li J. Li and Boyle, S.M. Dose and time dependent formation of benzo(a)pyrene metabolite DNA adducts in the spiny lobster, Panulirus argus. Marine Environ. Res. 34: 299-302, 1992
71. Kleinow, K., James, M.O. and Lech, J.J. Drug pharmacokinetics and metabolism in food-producing fish and crustaceans: Methods and examples. In D.H. Hutson, D.R. Hawkins, C.B. Struble and G. Paulson, editors. The fate of xenobiotics in food-producing animals. ACS Symposium Series, Number 503, pp98-130, Washington DC. 1992.
72. Ameer, B., James, M.O. and Saleh, J. Kinetic and inhibitor studies of acetaminophen and zidovudine glucuronidation in rat liver microsomes. Drug and Chemical Toxicology 15: 161-175,1992
73. Li C-L. J. and M.O. James. Glucose and sulfate conjugations of phenol, ß-naphthol and 3-hydroxybenzo(a)pyrene by the American lobster, (Homarus americanus). Aquatic Toxicology, 26: 57-71, 1993
74. Winston, G.W., James, M.O. and Jewell, C.S.E. In vitro studies of benzo(a)pyrene metabolism by representatives of the class crustacea. Polycyclic aromatic Compounds. 3 (Suppl): 1079-1086, 1993.
75. James, M.O., Hawkins, W.E. and Walker, W.W. Phase 1 and phase 2 biotransformation and carcinogenicity of 2-acetylaminofluorene in medaka and guppy. Aquatic Toxicology, 28: 79-95, 1994 .
76. Altman, A.H., Buono, R.J. and James, M.O. The effect of ecdysis on DNA of the hepatopancreas and green gland of the Florida spiny lobster (Panulirus argus). Comp. Biochem. Physiol. 107B: 419-426, 1994.
77. Barron, M.G. and James, M.O.: Oral bioavailability of single and multiple doses of sulfadimethoxine to the lobster, Homarus americanus. Xenobiotica 24: 921-932, 1994.
78. James, M.O., Altman, A.H., Li, C-L. J. and Schell, JD. Biotransformation, hepatopancreas DNA binding and pharmacokinetics of benzo(a)pyrene after oral and parenteral administration to the lobster, Homarus americanus. Chem.-Biol. Interact, 95: 141-160, 1995.
79. Boyle, S.M. and James, M.O. Cross-reactivity of an antibody to spiny lobster P4502L with microsomes from other species. Mar. Env. Res. 42: 1-9, 1996.
80. James, M.O., Kleinow, K.M., Tong, Z. and Venugopalan, C. Bioavailability and biotransformation of [3H]-benzo(a)pyrene metabolites in in situ intestinal preparations of uninduced and BNF-induced channel catfish. Mar. Env. Res. 42: 309-315, 1996.
81. James, M.O., Boyle, S.M., Trapido-Rosenthal, H.G., Smith, W.C., Greenberg, R. and Shiverick, K.T. cDNA and protein sequence of a major form of P450, CYP 2L in the hepatopancreas of the spiny lobster, Panulirus argus. Arch. Biochem. Biophys. 329: 31-38, 1996.
82. James, M.O., Altman, A.H., Morris, K., Kleinow, K.M. and Tong, Z. Dietary modulation of phase 1 and phase 2 activities with benzo(a)pyrene and related compounds in intestine but not liver of the channel catfish, Ictalurus punctatus. Drug Metab. Disp. 25: 346-354, 1997
83. Li, C.-L. J. and James, M.O. Pharmacokinetics of 2-naphthol following intrapericardial administration, and formation of 2-naphthyl-?-D-glucoside and 2-naphthyl sulphate in the American lobster Homarus americanus. Xenobiotica. 27: 609-626, 1997
84. Henderson, G.N., Yan, Z., James, M.O., Davydova, N. and Stacpoole, P.W. Kinetics and metabolism of chloral hydrate in children: identification of dichloroacetate as a metabolite. Biochem. Biophys. Res. Commun. 235: 695-698, 1997
85. Yan, Z., Henderson, G.N., James, M.O. and Stacpoole, P.W. Determination of dichloroacetate and its metabolites in human plasma by gas chromatography-mass spectrometry. J. Chromatog. B. 703: 75-84, 1997
86. James, M.O., Cornett, R., Yan, Z., Henderson, G.N. and Stacpoole, P.W. Glutathione-dependent conversion to glyoxylate, a major pathway of dichloroacetate biotransformation in hepatic cytosol from humans and rats, is reduced in rats by pretreatment with DCA. Drug Metab. Disp. 25: 1223-1227,1997
87. Li, C-L. J., and James, M.O. The oral bioavailability, pharmacokinetics and biotransformation of 9-hydroxybenzo(a)pyrene in the American lobster, Homarus americanus. Mar. Env. Res. 46: 505-508,1998
88. Boyle, S.M., Greenberg, R.M. and James, M.O. Isolation of CYP2L2 and two other cytochrome P450 sequences from hepatopancreas of the spiny lobster, Panulirus argus. Mar. Env. Res. 46: 21-24, 1998
89. James, M.O., Sikazwe, D.N. and Gadagbui, B. K.-M. Isolation of a pi class glutathione S-transferase from the intestinal mucosa of channel catfish, Ictalurus punctatus. Mar. Env. Res. 46: 57-60, 1998
90. Boyle, S.M., Popp, M.P., Smith, C.W., Greenberg, R.M. and James, M.O. Expression of CYP2L1 in the yeast Pichia pastoris. and determination of catalytic activity with progesterone and testosterone. Mar. Env. Res. 46: 25-28, 1998.
91. Kleinow, K.M., James, M.O., Tong, Z. and Venugapalan, C.S. Bioavailability and biotransformation of benzo(a)pyrene in isolated perfused intestinal preparations from control and ß-naphthoflavone-induced catfish. Env. Health Persp. 106: 155-166, 1998
92. James, M.O. and Boyle, S.M. Cytochromes P450 in crustacea. Comparative Biochemistry and Physiology Part C 121: 157-172,1998
93. Stacpoole, P.W., Henderson, G.N., Cornett, R. and James, M.O. Pharmacokinetics, metabolism and toxicology of dichloroacetate. Drug Metabolism Reviews 30: 499-539, 1998.
94. James, M.O., Yan, Z., Cornett, R., Jayanti, V.M.K.M., Henderson, G.N., Davydova, N., Katovich, M.J., Pollock, B. and Stacpoole, P.W. Pharmacokinetics and metabolism of [14C]-dichloroacetic acid in male Sprague-Dawley rats: Identification of glycine conjugates, including hippurate, as urinary metabolites of dichloroacetate. Drug Metabolism and Disposition 26: 1134-1143, 1998
95. Stacpoole, P.W., Henderson, G.N., Yan, Z. and James, M.O. Clinical Pharmacology and Toxicology of Dichloroacetate. Environmental Health Perspectives 106: supplement 4, 989-994, 1998.
96. Yan, Z., Henderson, G.N., James, M.O. and Stacpoole, P.W. Determination of chloral hydrate metabolites in human plasma by gas chromatography-mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 19: 309-318, 1999
97. Cornett, R., James, M.O., Henderson, G.N., Cheung, J., Shroads, A.L. and Stacpoole, P.W. Inhibition of Glutathione-S-Transferase Zeta and Tyrosine Metabolism by Dichloroacetate: A Potential Unifying Mechanism for its Altered Biotransformation and Toxicity. Biochem. Biophys. Research Commun. 262: 752-756, 1999
98. Gadagbui, B.K.M. and James, M.O. Differential expression of alpha-like GST isoforms in the catfish intestine. Marine Environmental Research 50:353-356, 2000.
99. Van den Hurk P. and James, M.O. Sulfation and glucuronidation of benzo(a)pyrene-7,8-dihydrodiol in intestinal mucosa of channel catfish (Ictalurus punctatus). Marine Environmental Research. 50: 11-15, 2000
100. Gadagbui, B.K.M. and James, M.O. Activities of affinity-isolated glutathione S-transferase from channel catfish whole intestine. Aquatic Toxicology, 49: 27-37, 2000.
101. Gadagbui, B.K.M. and James, M.O. The influence of diet on the regional distribution of glutathione S-transferase activity in channel catfish. Journal of Biochemical Toxicology, 14:148-154, 2000
102. Tong, Z. and James, M.O. Purification and characterization of hepatic and intestinal phenol sulfotransferase with high affinity for benzo(a)pyrene phenols from channel catfish. Arch. Biochem. Biophys. 376: 409-419, 2000
103. Doi, A., Lou, Z., Holmes, E. Li, C-L.J. Venugopalan, C., James, M.O. and Kleinow, K.M. Effect of micelle fatty acid composition and 3,4,3’,4’-tetrachlorobiphenyl (TCB) exposure upon intestinal [14C]-TCB bioavailability and biotransformation in channel catfish in situ preparations. Toxicological Sciences. 55: 85-95, 2000
104. Li, C.L. J. and James, M.O. Oral bioavailability and pharmacokinetics of elimination of 9-hydroxybenzo(a)pyrene and its glucoside and sulfate conjugates after administration to the American lobster, Homarus americanus. Toxicol. Sci. 57: 75-86, 2000.
105. James, M.O., Tong, Z, Rowland-Faux, L., Venugopal, C.S. and Kleinow, K.M. Intestinal bioavailability and biotransformation of 3-hydroxybenzo(a)pyrene in an isolated perfused preparation from channel catfish, Ictalurus punctatus. Drug Metabolism and Disposition, 29: 721-728, 2001.
106. James, M.O. Polychlorinated biphenyls: metabolism and metabolites. Proceedings of a workshop on “Recent Advances in the Environmental Toxicology and Health Effects of PCB” , L.W. Robertson and L. Hansen, eds, University of Kentucky Press, Lexington, 2001, pp 35-46.
107. Van den Hurk, P., Kubiczak, G.A., Lehmler, H.J. and James, M.O. Hydroxylated polychlorinated biphenyl as inhibitors of the sulfation and glucuronidation of 3-hydroxybenzo(a)pyrene. Environ. Health Perspect. 110: 343-348, 2002
108. Lou, Z., Johnson, J.V. and James, M.O. Intestinal microsomal metabolism of testosterone and progesterone by a 3-?-hydroxysteroid-oxidoreductase to the 3-?-hydroxy derivatives. J. Steroid Biochemistry and Molecular Biology, 82: 413-424, 2002
109. James, M.O. and Rowland-Faux, L. Hydroxylated polychlorinated biphenyls as poor substrates but good inhibitors of the glucuronidation and sulfonation of hydroxylated benzo(a)pyrene metabolites. Fresenius Environmental Bulletin, 12: 227-231, 2003.
110. Sugihara, N. and James, M.O. Binding of 3-hydroxybenzo(a)pyrene to hemoglobin and albumin. J. Biochem. Molec. Toxicol. 17: 239-247, 2003
111. Ammini, C.V., Fernandez-Canon, J., Shroads, A.L., Cornett, R., Cheung, J., James, M.O., Henderson, G.N., Grompe, M. and Stacpoole, P.W. Pharmacologic or genetic ablation of maleylacetoacetate isomerase increases levels of toxic tyrosine catabolites in rodents. Biochem. Pharmacol. 66: 2029-2038, 2003
112. James, M.O. Kleinow, K.M., Youbo Zhang, Ran Zheng, Liquan Wang and Laura R. Faux Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols. Marine Environmental Research, 58 (2-5), 343-346, 2004.
113. Sacco, J. and James, M.O. Glucuronidation in the polar bear (Ursus maritimus). Marine Environmental Research, 58 (2-5), 475-480, 2004.
114. Shroads, A.L., Henderson, G.N., Cheung, J., James, M.O. and Stacpoole, P.W. Unified gas chromatographic-mass spectrometric method for quantitating tyrosine metabolites in urine and plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 808(2):153-61, 2004
115. Wang, L.Q., Falany, C.N. and James, M.O. Triclosan inhibits the sulfonation and glucuronidation of several xenobiotic substrates in human liver fractions. Drug Metabolism and Disposition, 32:1162-1169, 2004.
116. Felitsyn N.M., Henderson G.N., James M.O., Stacpoole P.W. Liquid chromatography-tandem mass spectrometry method for the simultaneous determination of delta-ALA, tyrosine and creatinine in biological fluids. Clin Chim Acta. 350(1-2):219-30, 2004.
117. James, M.O., Lou, Z., Rowland-Faux, L. and Celander, M.C. Properties and regional expression of a CYP3A-like protein in channel catfish intestine. Aquatic Toxicology, 72: 362-371, 2005.
118. Wang, L-Q. Lehmler, H-J. Robertson, L.W., Falany, C.N. and James, M.O. In vitro Inhibition of human hepatic and cDNA-expressed sulfotransferase activity with 3-hydroxybenzo[a]pyrene by polychlorobiphenylols. Environmental Health Perspective, 113: 680-687, 2005
119. Sacco, J.C. and James, M.O. Sulfonation of environmental chemicals and their metabolites in the polar bear, Ursus maritimus. Drug Metab. Disp. 33(9): 1341-1348, 2005.
120. Wang, L.Q. and James, M.O. Sulfotransferase 2A1 forms estradiol-17-sulfate and celecoxib switches the dominant product from estradiol-3-sulfate to estradiol-17-sulfate. J. Steroid Biochemistry and Molecular Biology 96(5): 367-374, 2005
121. Hansen, L., Machala, M., Fischer, L., James, M., Hennig, B., Glauert, H., Narbonne, J.-F., van Bree, L., Schultz, T., Grevatt, P., Suk, W., Holoubek, I. and Robertson, L. Research needs identified at the Second PCB Workshop in Brno, Czech Republic, May 7-11, 2002. Toxicological & Environmental Chemistry 87(3): 261-265, 2005
122. Wang, L.Q. and James, M.O. Inhibition of sulfotransferases by xenobiotics. Current Drug Metabolism, invited review. 7: 83-104, 2006
123. Guo, X., Dixit, V., Liu, H., Shroads, A.L., Henderson, G.N., James, M.O. Stacpoole, P.W. Inhibition and recovery of rat hepatic glutathione S-transferase zeta and alteration of tyrosine metabolism following dichloroacetate exposure and withdrawal. Drug Metabolism and Disposition, 34: 36-42, 2006
124. Wang, L.Q. Lehmler, H.J., Robertson, L.W. and James, M.O. Polychlorobiphenylols are selective inhibitors of human phenol sulfotransferase 1A1 with p-nitrophenol as substrate. Chemico-Biological Interactions, 159:235-246, 2006
125. Doi, A.M., Lou, Z., Holmes, E., Venugopal, C.S., James, M.O. and Kleinow, K.M. Intestinal bioavailability and biotransformation of 3,4,3’,4’-tetrachlorobiphenyl in in situ preparations of channel catfish following dietary treatment with ?-naphthoflavone. Aquatic Toxicology 77: 33-42, 2006
126. Stuchal, L. Kleinow, K.M., Stegeman, J.J. and James, M.O. Demethylation of the pesticide methoxychlor in liver and intestine from untreated, methoxychlor-treated and 3-methylcholanthrene-treated channel catfish (Ictalurus punctatus): Evidence for roles of CYP1 and 3A family isozymes. Drug Metab. Disp. 34: 932-938, 2006
127. Wang, L.Q. and James, M.O. Sulfonation of 17?-estradiol and inhibition of sulfotransferase activity by polychlorobiphenylols and celecoxib in Channel catfish, Ictalurus punctatus. Aquatic Toxicology, 81: 286-292, 2007
128. Nichols, J.W., Erhardt, S., Dyer, S., James, M.O., Moore, M.M., Plotzke, K.P., Schulz, I.R., Segner, H., Thomas, K. and Weisbrod, A. Use of in vitro absorption, distribution, metabolism and excretion (ADME) data in bioaccumulation assessments for fish. Human and Ecological Risk Assessment. 13: 1164-1191, 2007
129. James, M.O., Sacco, J.C. and Faux, L.R. Effects of food natural products on the biotransformation of PCBs. Environmental Toxicology and Pharmacology. 25: 211-217, 2008
130. Blum, J.L., James, M.O., Stuchal, L.D. and Denslow, N.D. Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-a and beta-b by methoxychlor and its mono- and bis- demethylated metabolites in HepG2 cells. Journal of Steroid Biochemistry and Molecular Biology, 108: 55-63, 2008.
131. Blum, J.L., Nyagode, B.A., James, M.O. and Denslow, N.D. Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides) Aquatic Toxicology, 86: 459-469, 2008
132. James MO Stuchal LD, and Nyagode BA. Glucuronidation and sulfonation in vitro of the major endocrine-active metabolites of methoxychlor in the channel catfish, Ictalurus punctatus, and induction following treatment with 3-methylcholanthrene. Aquatic Toxicology 86: 227-238, 2008
133. Sacco, J. Robertson, L.W., Lehmler, H.J., Li, W. and James, M.O. Glucuronidation of polychlorobiphenylols and UDPGA concentrations in channel catfish liver and intestine. Drug Metab Disp. 36: 623-630, 2008
134. Shroads, A.L., Guo, X., Dixit, V., Liu, H.P., James, M.O. and Stacpoole, P.W. Age-Dependent Kinetics and Metabolism of Dichloroacetate: Possible Relevance to Toxicity . J. Pharmacol. Exp. Therap. 324: 1163-1171, 2008
135. Weisbrod, A.V., Sahi, J., Segner, H., James, M.O., Nichols, J., Schultz, I., Erhardt, S., Bonnell, M and Hoeger, B. The state of in vitro science for use in bioaccumulation assessments for fish. Environmental Toxicology and Chemistry in press.
Book Chapters
1. Bend, J.R. and James, M.O.: Xenobiotic metabolism by marine and freshwater species. In: D.C. Malins and J.R. Sargent, eds. Biochemical and Biophysical perspectives in Marine Biology. Vol. IV. pp 126 - 180, 1978.
2. Bend, J.R., James, M.O. and Pritchard, J.B.: Aquatic Toxicology. Chapter 13 in F.E. Guthrie and T.J. Perry, eds. Environmental Toxicology. Elsevier Press, New York, pp 172 - 180, 1980.
3. Pritchard, J.B. and James, M.O.: Metabolism and urinary excretion. In W.B. Jakoby, J.R. Bend and J. Caldwell, eds. Metabolic Basis of Detoxication. Academic Press, London, New York, 1982, pp 339 - 357.
4. James, M.O.: Biotransformation and disposition of PAH in Aquatic Invertebrates. In: U. Varanasi, ed. Metabolism of Polycyclic aromatic hydrocarbons in the Aquatic Environment. CRC Press. Boca Raton, 1989, pp 69 - 92.
5. James, M.O. and Kleinow, K.M. Trophic transfer of chemicals in the aquatic environment. In G. K. Ostrander and D. Malins, eds. Aquatic Toxicology: Molecular, Biochemical and Cellular Perspectives. Lewis Publishers, CRC Press, Boca Raton, 1994, pp 1-35.
6. James, M.O. Pesticide metabolism in aquatic organisms. In H. Börner, ed. Pesticides in Ground and Surface Water. Chemistry of Plant Protection Series. Springer-Verlag, Berlin. 1994, pp 153 - 189.
7. Kleinow, K.M. and James, M.O. Response of the teleost gastrointestinal tract to xenobiotics. In "Target Organ Toxicity in Marine and Freshwater Teleosts.", W. H. Benson and D.R. Schlenk, eds. Taylor and Francis, London, New York, 2001, pp 269-362
8. Schlenk, D., James, M.O., George, S., Gallagher, E., Willett, K., van den Hurk, P. and Kullman, S. Biotransformation in fishes. In “Toxicology of Fishes”, RT DiGuilio and DR Hinton, eds. CRC Press, Boca Raton, FL, 2008 Chapter 4.
